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Local Control of Cell Adhesion by Electrochemically Patterned Oligoethyleneglycol-Terminated Self-Assembled Monolayers
|Britta Vaske 1, Olaf Goldbaum 2, Christiane Richter-Landsberg 2, Gunther Wittstock 1|
1. Carl von Osseitzky University of Oldenburg, Department of Pure and Applied Chemistry, Oldenburg D-26111, Germany
Site directed adhesion of cells can be obtained by cultivation of adherent cells on patterned substrates containing cell-repellent regions and regions where cells adhere well. Of particular interest are substrates that can be dynamically altered as a cell cultivation experiment proceeds [1-3], for instance in order to introduce new cell types into previously cultured cells.
Patterned oligoethyleneglycol-terminated self-assembled monolayers (OEG SAMs) were created by microelectrochemically formation of Br2 [4-6]. These substrates were used to monitor local adherence of astrocyts derived from the brains of newborn rats  and oligodendroglia OLN-93 cells . These cells adhere without other specific substrate proteins and occupy selectively the manipulated OEG SAM surface.
Bromide is necessary as precursor for the microelectrochemical Br2 formation used for the modification of the OEG SAM. For the sequential introduction of cells into pre-existing patterns, it is important that adherent cells can withstand bromide concentrations in the culture medium. For that reason, the cytotoxicity of KBr addition to the culture medium (Dulbecco's Modified Eagle's Medium with 10 % fetal calf serum) was tested for OLN-93 cells between 25-100 mM KBr. The results show that these cells can tolerate 50 mM KBr concentration for the duration of 2 hours.
For sequential co-culture experiments on patterned substrates, two clonal cell lines OLN-93 are used: OLN-93 cells transfected with GFP protein and OLN-93 cells transfected with DsRed protein. The local attachment of the different cell populations could be detected by fluorescence microscopy like confocal laser scanning microscopy (CLSM).
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Presentation: Poster at SMCBS'2009 International Workshop, by Britta Vaske
See On-line Journal of SMCBS'2009 International Workshop
Submitted: 2009-08-31 11:06 Revised: 2009-10-27 15:17