Betamethasone is a glucocorticoid steroid with anti-inflammatory and immunosuppressive properties which can be used in treatment of asthma and itching. It may have some influence on chondrocyte proliferation. In this research, Polyvinyl alcohol nanosphere were prepared by oil in water emulsion method and the nanospheres were exposed on 1, 2 and 4 Freeze-Thawing (F-T) process including 24 hours freezing cycle at -25°C and 24 hours thawing at ambient temperature. Nanosphere production was confirmed by transmission electron microscopy and betamethasone release was analyzed by UV spectrophotometer at 245 nm. These data revealed the effect of the Freeze-Thawing process on drug delivery in which increasing the F-T cycles can decrease the rate of drug releasing. The effect of betamethasone concentration was also conducted on isolated human chondrocyte and MTT assay was utilized to assess the cell viability. The results showed that these biocompatible nanospheres can be used in sustained release of such drugs more than four month and it may be controlled by F-T cycles.

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