Silver nanoparticles/polystyrene nanocomposites were prepared via casting the solution of polystyrene in a mixture of carbon tetrachloride and acetone containing silver nanoparticles. Colloidal silver nanoparticles in acetone were synthesized by pulsed laser ablation (PLA) of pure bulk silver. Casting of the colloidal silver nanoparticles in a solution of polystyrene results in a yellowish transparent polymeric sheet. We study size and stability of particles in the polymer solvent (acetone). TEM images show rather spherical nanoparticles with mean diameter of 5 nm. Ag/PS nanocomposites were characterized by UV-VIS spectroscopy. In this study, we also investigated the antimicrobial activity of silver nanocomposites against Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) as a model for Gram negative and Gram positive bacteria. Bacteriological tests were performed in Luria bertani (LB) medium on solid agar plates and Nutrient broth in liquid system. These nanocomposites were shown to be an effective bactericide. Antibacterial tests were performed against E. coli and S.aureus, on LB agar plates containing different amount of nanoparticles. Our results showed at all these concentrations, the nanoparticles caused a growth delay of E. coli, increasing the concentration of nanoparticles increased this growth delay. Our results showed that PLA method can be used for Ag nanoparticle formation for antibacterial applications.

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