The successful treatment of patients and early preventing of a disease outbreak often require rapid and reliable pathogen detections. This study is a first step to achieve a cost effective and highly sensitive label-free biosensor based on the localized surface plasmon resonance phenomenon. Gold nano disks supported on a glass substrate with diameter of 100 nm and height of 70 nm are fabricated with the in house developed method, hole mask colloidal lithography (HCL). The diameter and height of the nano-disks are chosen so that the optical scattering and absorption of the nano disks match the visual light region 550 - 700 nm with an extinction maximum at around 600 nm.

The sensitivity and the electric field decay length of the nano disks are characterized by deposit thin layers of SiO₂ with different thickness. Biorecognition systems such as biotin - streptavidin and anti-prostate specific antigen antibody (anti-PSA) - PSA have been used to verify the biosensing application of such nano disks. With a noise level of 0,02 nm peak shift, the detection limit for the first layer of protein absorbed on the surface of nano disks is 10 ng/cm². A biosensing experiment with antibody and vaccine was also performed to demonstrate and simulate the pathogenic sensing ability of this kind of biosensors.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/15626 must be provided.