Many surfaces develop potentials when they contact solutions containing ionized substances. Very often, intermolecular attractions between the surface materials and the ionized substances are the basis of these "non-Faradaic" potentiometric phenomena. Until now, potentiometric sensing has made little use of the phenomenon to determine small organic ions and large biomolecules. Some reasons for this slow evolution are the lack of good theoretical models, and the lack of synthetic receptor molecules to attract small organics and large biomolecules to the specific surfaces used in potentiometry. Our group developed equipment to screen potentiometric electrode coatings for the determination of ionizable organics, using HPLC and CE. This equipment speeds up coating testing and allows one to perform measurements on less available but important (bio-)organics. Organic acids, oligonucleotides, amines, aminoalcohols, farmaceutical drugs, analgesic drugs, and proteins have been dealt with sofar. The electrode coatings that are most successful are the so-called "liquid membrane" types, based on PVC. When macrocyclic or podant host molecules are incorporated, the best recognized organic substances can be determined with extremely low detection limits. These host molecules are very efficient for the determination of a very important class of hydrophilic compounds. This approach is shown to work also for large biomolecules such as oligonucleotides. Another way of decreasing detection limits for the hydrophilic compounds is to increase the hydrophilicity of the electrode coating surface. For proteins, we used antibodies linked to the surface of conductive polymers as a recognition strategy. Applications of the electrode coatings will be shown in application for detection in HPLC and CE, as well as links will be given to DNA- and proteome chip technology.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/1253 must be provided.

1. Is it possible to study Interaction of molecules with sensor surfaces/components, using potentiometric “sensorgrams”?
2. Oligonucleotide and aptamer derivatized surfaces in potentiometric sensors: preliminary results
3. Surface Potentials: the Unexplored Way to Develop Sensors for Small Organic Ions and Large Biomolecules