Round Ti Al6 V4 alloy samples with the same surface texture were assayed in human bone marrow culture and in NMRI wild type mice. In the mice the samples were implanted both in the interscapular region and onto the temporal bone of the head after preparation of a rectangular periosteal flap which was then used to fix the sample subperiostally.
The new samples were examined by Scanning Electron Microscopy (SEM) and by Atomic Force Microscopy (AFM). New samples were enzymatically digested and again examined by SEM and AFM. After the exposition in cell cultures or the implantation in mice a fraction of the samples was prepared for immune histology and assayed in Confocal Laser Scanning Microscopy (CLSM) for the development/advent of cells of the lencocytic line or of the mesenchymal line respectively.
Another fraction was enzymatically digested, as described before, and examined by SEM and AFM. A third fraction of the animal implanted samples was examined in a mechanical testing device for the shear strength of the implant-bone-bonding.
Results:
There was practically no influence of the enzymatic digestion of new Ti Al6 V4 on the surface roughness of the samples.
Surface roughness has an influence on the development of cells of the lencocytic line on the samples in human bone marrow culture.
The surface roughness of Ti Al6 V4 samples decreased after implantation in the interscapular region as well as in the temporal headbone. This was especially true for roughness structures of the size of 20-40μm. The reduction surface roughness of samples implanted in the interscapular region was more pronounced in the submicron range. Surface roughness in the submicron range seems to be important for tissue-integration and coincides with the amount of gap bridging and mineralised tissue constructs between implant and bone.

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