Listeria monocytogenes, harmful bacteria present and transmitted in food, pose a serious threat to the health of consumers. The bacterium Listeria monocytogenes can reproduce at low temperatures and is therefore even more dangerous. A great number of legal regulations such as the Commission Regulation (CE) no. 2073/2005 on microbiological criteria for foodstuffs require the performance of tests to detect Listeria monocytogenes. For a foodstuff manufacturer the most important element is a short period of analysis, clarity and reliability of results.

The aim of the work was to evaluate the usefulness of a duplex PCR method to detect the bacteria Listeria monocytogenes in foodstuff samples. The results were confirmed by a classic method (PN-EN ISO 11290-1:1999/A1).

The starters flanking the specific sequences within genes coding the proteins participating in the process of Listeria monocytogenes pathogenesis were used for amplification. 16 reference strains were used in tests. At the same time the tests within the interlaboratory proficiency testing program (Quality in Microbiology Scheme, LGC Promochem) were performed and correct results were achieved every time. Testing also included foodstuff samples from outside suppliers and the analyses were considered tests confirming the results achieved with a classic method.

Tests to determine precision (sensitivity and specifity), repeatability and conformity of results are ongoing.

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