Introduction: Angiogenesis, a physiological process involving the formation of new blood vessels, is essential for many physiological processes, but is also fundamental in the certain pathological steps such as inflammation. Recently, it has been reported that human adipose tissue, active endocrine organ, contains a population of non-characterized cells, called stromal vascular fraction (SVF), which are able to differentiate to several lineages with the possible therapeutic potency. These cells may however take part in adipose tissue mass extension also due to participation in angiogenesis. Aim of the study was to confirm the proangiogenic properties of the SVF cells isolated from human subcutaneous adipose tissue.

Methods: SVF cells were isolated according to modified Hauner`s method and, after adaptation, cultured in proangiogenic, or proadipogenic medium. Cells were characterized by the expression of surface antigens (flow cytometry) and by the expression of genes characteristic for endothelial progenitor cells as well as for adipocytes performed using quantitative real-time PCR. The cell proliferation assay was performed using BrdU incorporation. Migration assay was assessed using the cell culture inserts. The tubules formation of GFP labeled SVF cells with not transfected HUVEC was prepared using the 3D matrigel model of angiogenesis. The differentiation to adipocytes was confirmed by the accumulation of lipid droplets stained with Oil-red-O.

Results: SVF cells are a heterogeneous population. Dependently to the used culture medium they may differentiate to adipocytes as well to endothelial-like cells respectively. This was documented by the pattern of gene expression. The proangiogenic SVF cells proliferate, migrate and form the capillary-like structure in the in vitro 3D model of angiogenesis. They also incorporate into tubules formed by HUVEC in this model. The longest tube formation was observed during co-culturing HUVEC with the GFP labeled SVF cells.

Conclusions: The differentiation of SVF cells towards endothelium is stimulated by the presence of human serum in the absence of adipogenic hormone mixture. It argues for the deep influence of the environment on the differentiation of adipose derived progenitors towards adipose versus angiogenesis promoting cells.

This work was supported by SC&CR (LSHB-CT-2004-502988), EU LIPGENE (FOOD-CT-2003-505944) and Polish MNiI (2P05A 132 28) projects.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/11124 must be provided.

1. Ranking of redox potential between CHD risk factors. Plasma ischemia modified albumin (IMA), hsCRP and Chlamydia pneumoniae seropositivity in obese/metabolic syndrome patients
2. Evaluation of genetic predisposition to insulin resistance by nutrient-induced insulin output ratio (NIOR).
3. Antioxidative effect of the short-term vitamin E and C combined supplementation in men with different risk of atherosclerosis.
4. CpG islands methylation induced by beta-carotene and arachidonic acid in human endothelial cells and its progenitors .
5. The chemotactic activity of beta-carotene in endothelial cell progenitors and HUVEC. The microarray analysis.
6. Animal models of human metabolic syndrome and pathological angiogenesis
7. Nutrient sensors, their polymorphism as the metabolic control and risk for cardiovascular disorders