The strong experimental evidences proved that HR-peroxidase is very sensitive detector of subtle changes in concentration of phenol, used as cofactor of peroxidase-oxidase reaction. The effects of low doses of phenol, prepared by successive dynamic dissolution in water or in 75% ethanol, on HRP activity were tested in systematic manner with colorimetric as well as luminometric methods. The sinusoidal shape of these relations showed the concrete phenol dilutions, which activated HRP (maximal points) and others with inhibiting doings (minimal points). These results were dependent on kind of dilutor. The dilutions with maximal values showed the higher relativity to hydrogen peroxide, whereas dilutions with minimal activity, corresponded mainly with oxidase type of reaction. Also the scan-kinetics revealed the distinct differences in shapes of UV-Vis spectra for two tested groups of phenol dilutions, which diverse also in Km values, lower for maximal and higher for minimal dilutions. The conception to recognize of HRP as a detector of dissolution of phenols is proposed.

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