The project NUTRASNACK (E.C. F.P.6 contract No FOOD-CT-2005-023044) has the main objective to produce by in vitro cultures secondary metabolites with health benefits, which can be used for fortification of some food products. On the of the key partner is ENERVIT^®, a well-known firm in the development of nutrition programs to enhance the performances of athletes and sportsmen. For the purpose of the project a number of plant species have been selected for the study of the possibility of commercial application of their secondary metabolites. This includes basil (Ocimium basilicum), dandelion (Taraxacum officinale), soybean (Glycine max), mint (Mentha piperica) and Leguminosae species (genus Trifolium).

For above species the conditions and protocols for the extraction of active principles need to be established. These protocols are aimed at the preparation of extracts for analytical purposes but also for commercial application for separation of large amounts of phytochemicals. For this purpose he extraction with different solvents and the purification of extracts by Reversed Phase C18 or  Amberlite XAD4 Solid Phase Extraction.

It was shown that in case of green samples two options should be considered. For samples used for analytical purposes the 70% EtOH should be a case of choice. For commercial purposes the water extract is more reasonable. Water extracts contain all active chemicals and allow to avoid chlorophyll presence in the extract. This substantially simplified the method of purification and enrichment.

In case of soybean the EtOH extract is necessary as isoflavones are not so readily soluble in water. Further purification can be achieved by SPE.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/10978 must be provided.

1. The effects of oxidative stress on the expression of PAI-1, t-PA,uPA and uPAR in vascular endothelial cells
2. LC-DAD-ESI/MSⁿ identification of active natural products from four plant species
3. The ultra-performance liquid chromatography (UPLC) analysis of phenolics in four plant species