Myofibroblasts (or smooth-muscle like cells) are now well known key-players of many physiological and pathological processes such as wound healing, hypertrophic scars formation, fibromatoses, stromal reaction, morphogenesis and differentation. These cells differentiate from fibroblasts under TGF-beta stimulation and are characterized by the expression of a- SMA (smooth muscle actin) what allows them to generate a tensile force required for wound contraction. Our studies were focused on the role of specific actin binding proteins and on their localization within migrating wound healing fibroblast. We have also paid attention to the changes in the organization of actin cytoskeleton and TGF-beta induced myofibroblasts alpha-SMA expression. Specific monoclonal antibodies against cofilin, gelsolin and actin were used to visualize proteins in fluorescent confocal microscope. Filamentous actin was stained with rodamine-conjugated phalloidin. We have observed pivotal changes in the localization of cofilin and gelsolin in fibroblasts migrating in the wound healing assay as well as in TGF-beta induced myofibroblasts. Our results underline a crucial role of these protein during dynamic changes of actin cytoskeleton in the processes of wound healing and others where a myofibroblast is involved.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/10946 must be provided.

1. The changes of actin cytoskeleton triggered by cofilin and gelsolin are correlated with high metastatic potential of human colon adenocarcinoma cells
2. Vinculin distribution accompanying to lumican-induced actin reorganization in human melanoma A375 cells