Significant decrease in the amount of large subunit (LS) of Rubisco were found to occur in response to an acclimation (4-6 days) to low irradiance of Arabidopsis plants grown at moderate irradiance. When the lysates of intact chloroplasts were incubated in vitro in darkness the disappearance proceeded so that less than 10% of initial amount of LS Rubisco remained after 6h of the incubation. The low irradiance-dependent disappearance in vitro was ATP-independent, had its optimum at pH 7.5 and required 3mM Mg²⁺. Studies using typical inhibitors of various classes of proteases indicate that the disappearance is an enzymatic phenomenon and that members of two classes of chloroplast proteases enzymes may be responsible.

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