Breast cancer is a major cause of morbidity and mortality among women worldwide especially in middle age and growth factors has been found to play an important role in the etiology and progression of this disease. Several proteins of the Renin–Angiotensin–Aldosterone system (RAS) have been implicated in the processes of growth promotion or inhibition and are found present both in normal and cancerous breast tissues. Breast epithelial cells express components of the renin angiotensin system and studies suggest that these may be altered in disease progression. Angiotensin II (AngII), a peptide of the renin-angiotensin system, can not only regulate the water-electrolytic balance and control the arterial blood pressure but it can also influence the cellular proliferation. It is known, that this peptide influences not only the hyperplasia of the smooth muscle of the blood vessels but also the cells of the anterior pituitary lobe and the cells of the adrenal gland and how the latest research has shown, it influences also the proliferation of some tumor cellular lines. The aim of the study was to find out if the AngII can modulate the cell growth of two cellular lines of the breast tumor MCF7 (hormon-dependent line) and MDA-MB-231 (hormon-independent line). The influence of the peptide on the cell proliferation was assessed using standard MTT assays and changes of the tyrosine kinases activity (enzymes which are directly engaged in the cell proliferation). The influence of the 17-β-estradiol on the activity of the AngII has also been estimated. AngII 10^-9M, 10^-10M and 10^-11M has strongly slowed down the activity of the tyrosine kinases and cell proliferation in the MDA-MB-231 line. AngII alone in all concentration has very weakly influence on the activity of the tyrosine kinases in the MCF7, but in the presence of estradiol in the concentration 10^-6 this peptide strongly inhibited examined enzyme. The results show that AngII can be the next factor, which modulates the proliferation of the cellular lines of the breast tumor.

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