Nucleolar organizer regions (NORs) are useful tool in many kinds of researches from evolutionary analysis to tumor pathology. The aim of the present study was the evaluation of AgNORs (silver deposits) presence and size on the metaphase chromosomes obtained after lymphocytes cultures to describe cell stress. Oxidative stress was induced by hydrogen peroxide (H₂O₂) treatment at concentrations ranging from 1 to 1000 μM. The quantitative analysis of AgNOR proteins on cytogenetic preparations were obtained by the “counting method” and “the morphometric method”. Statistical analysis of the data was performed using t-Student’s test. These examination revealed that degree of transcriptional activity of rDNA was reduced with increasing hydrogen peroxide concentration. In conclusion, AgNOR staining as simply and quick method may be complement to other methods like comet assay, micronuclei assay or SCE especially during testing compounds at low concentration.

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