The CacyBP/SIP protein was originally discovered as a S100A6 (calcyclin) target (Filipek and Kuźnicki, 1998) and later as a Siah-1 interacting protein (Matsuzawa and Reed, 2001). CacyBP/SIP also interacts with some other members of the S100 family (Filipek et al., 2002a) and with the Skp1 protein. The function of CacyBP/SIP is not clear at present although there are some results published suggesting that it can be a component of the SCF ubiquitin ligase (Matsuzawa and Reed, 2001) and that it might play a role in cell differentiation (Au et al., 2006). In particular, it has been shown that during differentiation of rat neonatal cardiomyocytes CacyBP/SIP is up-regulated. Since CacyBP/SIP is highly expressed in neuroblastoma NB2a cells (Filipek et al., 2002b), in this work we examined the influence of CacyBP/SIP on differentiation of these cells. We found that in differentiated cells, the level of CacyBP/SIP, established by western blot, was higher than in undifferentiated ones. Immunofluorescence experiment showed that CacyBP/SIP staining becomes very intensive in the cellular processes formed upon cell differentiation. This suggests that CacyBP/SIP plays a role in differentiation of neuroblastoma NB2a cells and points to a possible function of this protein in differentiation of brain neurons under physiological and pathological conditions. The role of CacyBP/SIP in a signaling pathway involved in the differentiation process is under investigation in our laboratory.

This work was supported by the State Committee of Scientific Research grant to A.F (2 P04A 01030) and statutory funds from the Nencki Institute of Experimental Biology. G. Schneider is a recipient of a scholarship from the President of the Polish Academy of Sciences, and E. Kilanczyk and J. Kuznicki are recipients of a stipend from the Foundation for Polish Science.

Au K.W., Kou C.Y., Woo A.Y., Chim S.S., Fung K.P., Cheng C.H., Waye M.M., Tsui S.K., (2006) J Cell Biochem 98, 555-66

Filipek A., Kuznicki J., (1998) J. Neurochem. 70, 1793-1798

Filipek A., Jastrzebska B., Nowotny M., Kuznicki J., (2002b) J. Biol. Chem. 277, 21103-21109

Filipek A., Jastrzebska B., Nowotny M., Kwiatkowska K., Hetman M., Surmacz L., Wyroba E., Kuznicki J., (2002a) J. Biol. Chem. 277, 28848-28852

Matsuzawa S., Reed J., (2001) Mol. Cell 7, 915-926

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