Ionizing radiation is used in treatment of at least half of tumor types. The exposure to ionizing radiation, similarly to other stressing factors, leads to activation of signaling pathways, which determine the general cellular stress responses; activation of DNA repair mechanisms, inhibition of cell cycle, change of genes expression profile or cell death.

In our investigations we analyzed the influence of ionizing radiation on gene expression in human cell lines: melanoma Me45 and leukemia K562. Cell cultures were exposed to 4 Gy dose of ionizing radiation and total RNA was isolated at different times after irradiation (immediately, 12, 24 and 36 hours) and used to microarray and Real-time PCR analysis. The data from microarrays (Affymetrix) were normalized and analyzed with FatiGO tool. The KEGG metabolic pathways that showed the prevailing decrease in gene expression immediately after irradiation were neuroactive ligand-receptor, cytokine-cytokine receptor and calcium signaling pathways. Among the pathways whose gene expression increased were: oxidative phosphorylation, ribosome, ATP synthesis and proteasome. The expression of some genes belonging to these metabolic pathways was checked by Real-time PCR. Different methods of PCR analysis were applied and their influence on the results will be discussed.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/10863 must be provided.

1. Analyses of lymphocyte radiosensitivity in vitro for identification of predictive biomarkers of early response to radiotherapy in head and neck cancer patients
2. Polymorphism of DNA repair genes as a cancer risk factor; comparison between head and neck squamous cell carcinoma and colon cancer