No considerable differences were found in the total vitamin B₁ pools in studying the metabolisms of thiamine as well as its di- and triphosphate esters in the blood, liver and brain of control and stressed adult male albino rats. After stress, the decrease in the concentration of total thiamine diphosphate (TDP), the vitamin B₁ coenzyme form, did not reach a significant level. However, in differentiated determination of free and protein-bound TDP a marked and significant reduction of free coenzyme content was detected. Along with the diminished free TDP found after immobilization stress, the thiamine triphosphate (TTP) level was also decreased, its brain and heart concentrations being almost half reduced. This occurred in combination with the raised activity of specific thiamine triphosphatase catalyzing TTP degradation. In its turn, the second hydrolytic enzyme, thiamine diphosphatase, did not intensify TDP splitting. The comparison of the activities of the TDP- and TTP-synthesizing systems, thiamine kinase and thiamine diphosphate kinase, indicated that the stress factors changed the rate of TTP formation more pronouncedly as opposed to that of TDP synthesis, which was characteristic of all the objects under study. The preliminary administration of thiamine physiological doses to stressed animals considerably levelled the alterations noted. The antistress vitamin B₁ effect may be due to its involvement in redox processes directed to maintenance of cellular thiol levels.

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