Several families of protein kinaases are involved in plants stress signaling. Among them are kinases common for all eukaryotes (e.g. kinases consisting MAPK cascades), as well as kinases specific for plants. In our laboratory we have identified, cloned and partly characterized two specific for plants protein kinases (ZmCPK11 and NtOSAK) which are involved in abiotic sress signal transduction.

In maize leaves the activity of the expression of calcium dependent protein kinase classified as ZmCPK11 is activated by wounding (Szczegielniak et al., 2005). Activity and the level of ZmCPK11 transcript is also increased in non-injured neighboring leaves, what suggests that the maize protein kinase in involved in a systemic response to wounding. The recent evidences demonstrate that ZmCPK11 is the integral component of wound induced jasmonic acid dependent stress response.

In tobacco cells (BY-2) was identified a protein kinasse activated rapidly in response to osmotic stress (Mikołajczyk et al., 2000). The kinase, named as NtOSAK (Nicotiana tabacum osmotic stress-activated protein kinase), belongs to SNF1-related kinase 2 (SnRK2) subfamily. Phosphorylation is involved in activation NtOSAK. We mapped the regulatory phosphorylation sites of NtOSAK by mass spectrometry (Burza et al., 2006). In BY-2 cells subjected to osmotic stress Ser-154 and Ser-158 is phosphorylated. It correlates with NtOSAK activity, what indicates that NtOSAK is regulated by reversible phosphorylation of these residues in vivo.

Burza A.M., Pękala I., Sikora J., Siedlecki P., Małagocki P., Bucholc M., Koper L., Zielenkiewicz P., Dadlez M., Dobrowolska G. (2006) Nicotiana tabacum Osmitic Stress-activated Kinase is regulated by phosphorylation on Ser-154 and Ser-158 in the kinase activation loop. J. Biol. Chem. 281: 34299-34311

Mikołajczyk M., Awotunde O.S., Muszyńska G., Klessig D.F., Dobrowolska G. (2000) Osmotic stress induces rapid activation of a Salicylic Acid-Induced Protein Kinase and a homolog of protein kinase ASK1 in tobacco cells. Plant Cell 12: 165-178

Szczegielniak J., Klimecka M., Liwosz A., Ciesielski A., Kaczanowski S., Dobrowolska G., Harmon A.C., Muszyńska G. (2005) A Wound Responsie and Phospholipid-Regulated Maize Calcium-Dependent Protein Kinase. Plant Physiology 139: 1970-1983

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1. Effects of wounding of maize leaves on the contents of 5-methylcytosine in DNA