The synthetic human IFN-β structural gene was fused to synthetic ubiquitin sequence and adapted to the E.coli codon usage. We compared the stability of the same IFN-β sequence in two types of vectors: pT7RS with T7 promoter and pDB with deo P1P2 promoter. E.coli laboratory strains: BL21 DE3, NM522 and DH5α were used in transformation procedure. The stability was confirmed for over 40 generations in the E.coli BL21DE3 strain which was transformed with pT7RSIFNβ vector. The results were demonstrated by comparing two parameters: the level of recombinant protein expression and the ratio of antibiotics resistant clones in general population. Identification of expression level was analysed by SDS- PAGE. With the use of the plasmid/host combination we could directly increased the ratio on expressions level.

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1. New retrons isolated from clinical E. coli strains