Peanut stunt virus (PSV) is a member of the genus Cucumovirus in the family Bromoviridae. It is one of the most important pathogens of the legumes all over the world. Its genome consists of positive single strand RNAs: RNA1 and RNA2 encode 1a and 2a protein, respectively, RNA3 encodes 3a movement protein and a coat protein translated from the subgenomic RNA4. In some strains along with their genomic and subgenomic RNAs may be packed fifth component designated as satellite RNA (satRNA).

The proteins 1a and 2a are the compounds of the viral replicase complex. The N-terminal region of the PSV 1a protein contains methyltransferase domain involved in capping of genomic strands, whereas the C-terminal encodes helicase domain. The PSV 2a protein has the characteristic conserved motif of RNA-dependent RNA polymerase (RdRp).

In our study we have analysed the 2a polymerase protein sequence of chosen Polish strains of PSV from celery (PSV-Ag) and pea (PSV-G) and we have compared them to other known sequences from the genus Cucumovirus.

RNA from purified virus particles was isolated by phenol/SDS extraction followed by ethanol precipitation. Then we have carried out the RT-PCR reactions to amplify the 2a protein of examined PSV strains. The specific primers were designed on the basis of known sequences of PSV RNA2. The products of amplification were subsequently cloned, sequenced and analysed in BLAST. The phylogenetic analysis was performed in PAUP 4.0 software. The comparison of 2a protein nucleotide sequences has shown the highest similarities (84%) to PSV-ER and PSV-J belonging to subgroup I of PSV. Furthermore, the amino acid identities with mentioned above PSV strains range from 86-87%, whereas the similarities 90-92%. The analysis of RdRp domain shows also high amino acid identities to other Cucumovirus ranging from 79-96%. Obtained data confirms our previous research on coat protein sequences of Polish isolates which indicated the high homology with subgroup I of PSV and their close genetic relation to each other.

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