The presense of CBP proteins (Cytokinin Binding Protein) was demonstrated in many of plant species, algae and bacterias. The investigations resulted in isolation of a cytoplasmal mung bean protein with high affinity to these phytohormones (Fujimoto et al.,1998). The protein mass was determined at 17 kDa (Vigna radiata cytokinin specific binding protein).

Searching of a protein data bases and comparison of amino acid sequences of PR-10 (PR, pathogenesis related proteins) and CSBP proteins showed less than 20% identity. However, on the ground of their secondary structures’ similarities it was proved that both protein groups have analogous construction of ß sheets and α helics (Pasternak et al., Plant Cell 18(10):2622-34, 2006). However, phylogenetic analysis clearly distinguish CSBP proteins as a separate subclass.

Two homologue nucleotide sequences were found in yellow lupine expression library:Llcsbp1 and Llcsbp2. These homologues differ in terms of sequences and number of coding amino acids: LlCSBP1-158 aa, LlCSBP2-155 aa.

The goal of this study was quantitation of Llcsbp1 and Llcsbp2 gene expression level in variuos organs of yellow lupine, using reverse transcriptase real-time PCR method with SYBR Green I as a detection format. The obtained quantitative results were normalized towards ubiquitin expression level as a control gene. The specificity of the reaction was confirmed by melting curve analysis and agarose gel electrophoresis for all fragments of the investigated genes. Finally, statistical tests were performed.

The results showed significantly higher Llcsbp1 gene expression level than Llcsbp2 in such organs as young and old leaves, pods and roots. The situation appeared to be different only in seeds, where the expression level of Llcsbp1 gene was by half lower than the expression level of Llcsbp2.

This experiment confirms diverse and low levels of expressed both homologue genes in organs under investigation. That suggests, they might be involved in signal transduction induced by cytokinins, during various physiological processes.

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