LCMS-QIT-TOF: High-Speed Liquid Chromatograph Mass Spectrometer for structural analysis.

Dr. Marek Szklarczyk

SHIM-POL, ul Lubomirskiego 5, 05-080 Izabelin, Poland

Dr. Marcus Mreyen, Dr. Alan Barnes

Shimadzu Europe GmbH, Albert-Hahn-Str. 6-10, D-47269 Duisburg, Germany.

There are several types of LC/MS instruments used in the different laboratories. The single quadrupole and triple quadrupole instrumets are primarily used for quantitative analysis, while the ion trap, LC-TOF and Qq-TOF instruments are basicly used for qualitative analysis. Triple quadrupoles, the excellent instruments for quantitative analysis, unfortunately falter in terms of mass accuracy and resolution. Ion traps are excelled in structural analysis due their ability to perform MSⁿ analysis, however, their mass accuracy and resolution, like for a triple quadrupole MS is strongly limited. The Qq-TOF instruments features excellent resolution and mass accuracy, but because their construction they do not have ability to perform higher stage analysis then MS³ what makes limited their application in structural analysis.

Shimadzu developed a new type of hybrid mass spectrometer, the LCMS-QIT-TOF, which possesses both the MSⁿ ability of an ion trap, and the excellent resolution and mass accuracy of a TOF analyzer. This hybrid instruments opens new doors to the prediction of elemental composition and structural analysis. The special construction of ion trap, four parts, greatly increases accurate MSⁿ fragmentation. The so call Quadrupole (four parts) Ion Trap (QIT) is in the unique linear arrangement with TOF analyser what increases speed of analysis and allows to avoid trap saturation. It allows to get qualitative information within a limited HPLC peak elution time.

In this presentation we will present the unique construction of the LCMS-QIT-TOF instrument pointing the innovative octopole lens construction. The several chemical and biochemical applications will be presented, e.g. for flavonoids and proteins. The application of LCMS-QIT_TOF instrument for analysis of small and large molecules will be shown. The step by step multi stage, MSⁿ, analysis will be explained.

The universality of new instrument makes possible its application in qualitative analysis, structural and metabolite ID studies, impurity analysis, proteomics and biomarker studies.

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