Biomineralization is general process by which inorganic salt crystals are formed in biological environments. In order to study the mechanisms of the biomineralization, polyelectrolyte multilayers film were used to induced nucleation and growth of inorganic crystals. Nucleation and growth are controlled by specific interactions between mineral crystals and organic or inorganic molecules. Still there are many unanswered questions especially concerning the essential components involved in the initiation of mineralization and in the regulation of crystal growth. Alternate layer by layer adsorption is a powerful and convenient technique for preparation of organized molecular films. This surface may precisely be an appropiate substrat for cristal nucleation.
The aim of this study was to investigate the influence of polyelectrolyte films to calcium phosphate nucleation. Two situation where studied: when the polyelectrolyte surface is formed by polyallyamine (PAH) positive surface and polystyrenesulfonate (PSS) as negative surface. The results were also compared with bare surface. The proces of nucleation of calcium phosphate was followed in situ using Optical Waveguide Lightmode Spectroscopy (OWLS) and AFM liquid cell. This technique allows focusing on the process occurring directly at the surface. Crystal morphology was determine by scanning electron microscopy (SEM). The SEM images shows that three dimensional crystalilites are formed on the PSS terminated film and two dimensional on the PAH terminated film.
Acknowledgement:
The research was carried out under 5th European Framework Programme:: Surface Improvement On Metal Implants - SIMI, Project G5RD- 2000-00423.

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