The use of biocatalysts in SC-CO₂ has received widespread attention in recent years because of many advantages. Biocatalysts demonstrate substrate specificity under mild reaction conditions and SC-CO₂ has several advantages over liquid solvents such as high solute diffusivities and low viscosity, which accelerate mass transfer-limited enzymatic reactions.

Two types of enzymes (lipase and glucose 2-oxidase from Ganoderma lucidium) will be tested under SC-CO₂ from the point of view of enzyme stability and enzyme specificity. The effects of molar ratio of the substrates, water content, enzyme load, pressure and temperature, time course and substrate flow rate on both the rate of enzyme reaction and product distributions will be estimated.

The kinetic, thermodynamic and transport phenomena have a pronounced influence on enzyme catalysed reactions, and their understanding is a key step in the modelling and design of novel processes which operate at high pressures and involve phase and chemical equilibrium. Thus, particular attention will be given to studying and modeling the kinetics of the enzyme-catalysed reactions, to modeling the simultaneous phase and chemical equilibrium.

Acknowledgement: Czech part of the project (D30.001) founded by the Ministry of Education of the CR..

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/6219 must be provided.

1. Enzymic catalysis in supercritical carbon dioxide
2. Effect of physico-chemical parameters on biocatalysis of glucose 2-oxidase from Coriolus versicolor at high pressure