Viscum album L. (Viscaceae) known as mistletoe is evergreen semi-parasitic plant which grows on various hosts.  Mistletoe preparations have multiple applications, e.g. as antiatherosclerotic, sedative, anticancer and analgestic remedies. Moreover, ethanol extract is used as a hypotensive drug. The anti-inflammatory effect was also reported in literature [1,2].

Pentacyclic triterpenes belonging to common secondary plant metabolites have strong anti-inflammatory activity and can influence on biological  properties of V.album [3].  

            In our paper, the content of two triterpenes: olanolic (OA) and betulinic (BA) acid in V.album  of different origin was determined by HPLC-DAD method. The separation was achieved on RP 18 column at 1ml/min^-1 flow rate and at temperature of 10°C. Acetonitrile, water and phosphoric acid (80:20:0.5 v/v/v) were used as a mobile phase. The established equation  of calibration curve (y = 62898x+17711 for BA and y = 55925x+126773 for OA) and the other validation parameters: correlation coefficient (r ˃ 0.9999) and precision (RSD values ranged from 0.01% to 2.2%) were found to be satisfactory for the proposed method. As showed our investigation, the amount of both acids differed significantly depending on host. It ranged from 0,36 ng/g (host: Sorbus aucuparia, Malus John Downie) to 1.57 ng/g (Tilia cordata) for BA and from 3,11 ng/g (Malus John Downie) to 9,56 ng/g (Malus Jonathan) for OA.

References:

[1] S. I. Vicaş, D. Rugină, L. Leopold, A. Pintea, C. Socaciu: Not. Bot. Hort. Agrobot. Cluj. 39, 48-57 (2011).

[2]  A. Panossian, A. Kocharian, K. Matinian, E. Amroyan, E. Gabrielian, Chr. Mayr,

H. Wagner: Phytomedicine 5, 11-17 (1998).

[3] J. Liu: J. Ethnopharm. 100, 92-94 (2005).

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