Vitamin C (ascorbic acid, 2-(1,2-dihydroxyethyl)-4,5-dihydroxyfuran-3-one, AA) is a water-soluble organic compound essential in many biological process e.g., it protects the immune system, enhances iron bioavailability, reduces cholesterol levels and is necessary in the formation and maintenance of collagen. Moreover, ascorbic acid is strong antioxidant and it is extensively used for the prevention and treatment of the common cold, some mental illnesses, and cancer [1-3]. The main disadvantage of ascorbic acid is relatively low stability of water solution.

The aim of our research was investigation of ascorbic acid stability depending on time, temperature, concentration. and type of stabilizer. The analysis was performed by HPLC-DAD method in reversed phase C-18 system using water with addition of trifluoric acid at concentration of 0.025% as a mobile phase.

References:

[1] O. Arrigoni, C.F. Tullio, Ascorbic acid: more than just an antioxidant, Biochim. Biophys. Acta 11569 (2002) 1-9.

[2] R. Aguilar, M.M. Dávila, M.P. Elizalde, J. Mattusch, R. Wennrich, Capability of a carbon-polyvinylchloride composite electrode for the detection of dopamine, ascorbic acid, and uric acid, Electrochim. Acta 49 (2004) 851-859. 

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