Electron paramagnetic resonance (EPR) spectroscopy was used to examine free radicals in thermally treated acidum boricum (AB). Thermal treatment in hot air as sterilization process was tested. Sterilization is expected to exterminate microorganisms in acidum boricum [1]. Conditions of thermal sterilization are described in the pharmaceutical Norms [2]. It is expected that thermal sterilization related breaks of chemical bonds and free radicals formation in acidum boricum samples depend on temperature. Our previous works reported formation of free radicals in organic pharmaceutical substances at high temperature [3-5].The aim of this work was to determine concentration and free radical properties of thermally sterilized acidum boricum, which is used as a 3% solution to wash infected wounds and burns.[1].

There is no literature information about free radicals in AB samples. EPR results for AB were compared to results obtained for the others thermally sterilized drugs [6].

EPR analysis for AB was done 15 minutes, 2, 8, 10, 13, 16, 22, 32 and 40 days after sterilization. EPR measurements were done at room temperature. EPR spectra were recorded in the range of microwave power of 2.2-70 mW. g-Factor, amplitudes (A), integral intensities (I), and linewidth (ΔB_pp) of the spectra were determined. The shape of the EPR spectra was analysed.

EPR spectra were not obtained for the non heated AB. As was expected free radicals do not exist in the original AB sample. EPR spectra were detected for the all thermally sterilized samples. The lowest free radicals concentration was found for the AB sterilized at temperature 170 ^oC during 60 minutes. Free radicals concentrations in sterilized AB change during storage as the result of oxygen interactions with the samples.

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1. EPR studies of thermally sterilized Vaselinum Album
2. EPR studies of free radicals in A-2058 human melanoma cells treated by valproic acid and 5,7-dimethoxycoumarin
3. Effect of Cd(II) on free radicals in DOPA-melanin tested by EPR spectroscopy
4. Examination of free radicals in melanins isolated from A-375 cells exposed on valproic acid and cisplatin by EPR spectroscopy