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EPR studies of thermally sterilized Vaselinum Album

Paweł Ramos ,  Barbara Pilawa 

Department of Biophysics, Medical University of Silesia, Jedności 8, Sosnowiec 41-200, Poland

Abstract
Sterilization process in pharmacy is preformed to kill microorganisms in vaselinum album and as the result to rise the safety of the pharmacotherapy [1]. The popular method of sterilization is the thermal sterilization with hot air circulation in the vaselinum album samples [1]. The parameters of thermally sterilization  is determined by pharmaceutical norms[2-5]. Thermal sterilization should not produce the high amount of free radicals in the vaselinum album and their chemical structure should not be destroyed. Free radicals in the sterilized vaselinum album be studied by electron paramagnetic resonance (EPR) spectroscopy. Free radicals and chemical structure of thermally sterilized vaselinum album were examined in this work. The aim of this study is to find optimal conditions of thermal sterilization of the analysed vaselinum album.
Sterilization was performed at temperatures: 160o C (120 minutes), 170o C (60 minutes), and 180o C (30 minutes). The electron paramagnetic resonance spectra were measured at room temperature by the use of an X-band (9.3 GHz) EPR spectrometer of RADIOPAN Firm (Poznań) and the system of numerical acquisition of JAGMAR Firm (Kraków). g-Factors, amplitudes, integral intensities, and line widths of the EPR spectra were analysed. Free radicals concentrations were determined. The effect of microwave power on the line shape and parameters of the EPR spectra was presented.
The original vaselinum album sample was diamagnetic. EPR spectra were obtained for the thermally sterilized vaselinum album. The parameters of the EPR spectra, microwave saturation of the lines, and free radicals concentrations in the heated samples depend on the conditions of sterilization. It was shown that EPR method may be used to optimize the thermal sterilization process of vaselinum album and conditions of its storage.

References:

1. S. Janicki, A. Fiebig, Farmacja stosowana, PZWL, Warszawa, 2008.
2. Farmakopea Polska IX. PTFarm: Warszawa 2011.
3. PN-EN 552. Polski Komitet Normalizacyjny, Warszawa 1999.
4. PN-EN 552. Polski Komitet Normalizacyjny, Warszawa 2002.
5. ISO 11137. International Organization for Standarization, Genewa 2006.

 

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Presentation: Oral at IX Multidyscyplinarna Konferencja Nauki o Leku, by Paweł Ramos
See On-line Journal of IX Multidyscyplinarna Konferencja Nauki o Leku

Submitted: 2014-03-15 17:01
Revised:   2014-05-02 18:53