Synthesis of electroactive films of Prussian Blue: improvement of selectivity and stability of the advanced H2O2 transducer.

Anastasiya Borisova 1Aleksandr G. Chuchalin 2Eldar H. Anaev 2Arkady A. Karyakin 1

1. Chemistry Department, Moscow State University, Leninskie Gory, 1-3, Moscow 119992, Russian Federation
2. Pulmonology research institute, Moscow 105077, Russian Federation

Abstract

The problem of monitoring of low levels of hydrogen peroxide is required in medicine, environmental control and in industry. There are known different methods of such detection, but electrochemical analysis is the most sensitive, selective and the cheapest method.
The construction of electrochemical sensor for hydrogen peroxide is based on advanced electrocatalyst for hydrogen peroxide reduction (Prussian Blue). The application of Prussian Blue modified electrodes for sensors and biosensors construction has been shown for the first time in our group [1]. To start mass production of sensors it is necessary to apply planar electrodes based on screen-printed technology as well as to simplify the procedure of electrodes modification by nano-scaled layers of Prussian Blue. We have been developed the new method of chemical deposition of nano-scaled films of Prussian Blue allowed to increase the reproducibility of sensors and to lower their cost. Analytical characteristics of developed sensors tested in FIA are: linear range of hydrogen peroxide detection 10-7 – 10-3 M, low response time (<1 min), operational stability >1000 measurements. Significant increase of the stability of Prussian Blue can be achieved by covering electrode by polymeric membrane (Nafion®). Under the constant flow of hydrogen peroxide with concentration 10-4 M sensor holds 100 % stability during more than 1 hour. Combining precipitation Prussian Blue with some organic polymers as polyaniline, polypyrrole and different substituted polypyroles make it possible to improve the analytical characteristics of sensors. The new fields of application of developed sensor for hydrogen peroxide have been defined. Sensor could be applied in clinical diagnostics for oxidative stress detection. Sensor has been used for development of novel method of non-invasive diagnostics of condensate of expired air in pulmonology.

[1] Karyakin A.A. (review). Encyclopedia of Sensors (Am. Sci. Publ.) 1 (2006) 329-351.

Acknowledgements. Financial support through RFFI 06-03-33013-a, RFFI-07-03-12211-innovation, grant of Federal Agency for Science and Innovation (2007) and IINTAS innovation 05-000007-429 and ISTC 32-09 are greatly acknowledged.

 

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Presentation: Poster at SMCBS'2007 International Workshop, by Anastasiya Borisova
See On-line Journal of SMCBS'2007 International Workshop

Submitted: 2007-10-05 14:29
Revised:   2009-06-07 00:44