Peripheral blood monocytes are populations of cells of high importance for resistance of the body. When migrating to inflammation sites, they constitute populations cells able to phagocyte and to present an antigen to other immune cells.

The artificial neural network analysis is a method of data analysis which is to emulate the brain’s way of working. Neural networks are easier in use than traditional statistical methods, since they construct models needed by the user themselves.

Methods: Monocytes were isolated from peripheral blood of 31 healthy donors by the lymphocyte separation media density gradient and then cultured for 7 days in the RPMI medium with 10% autologous serum. Phagocytosis was determined using the PHAGOTEST kit, and then analysed by flow cytometry (FACSCalibur, Becton Dikinson). The percentage of monocytes/macrophages engaging in phagocytosis and the percentage of cells presented the CD 68 antigen were determined. The fatty acids content in blood was measured by gas chromatography (Perkin Elmer 8500). The cholesterol fractions and the triacylglycerol (TG) content were measured in the fasting plasma with commercial test kits (bio-Merieux, France). Phosphorylation of the ERK1/2 kinase and PPAR-γ2 was measured by the western blot method. The neural networks were used as a calculation method with the SNN (Statistica Neural Networks) STATSOFT software package.

Results: The content of arachidonic acid (AA) in the blood was a factor strongly determining monocyte/macrophage phagocytosis. At the same time, this feature was also strongly determined by plasma concentration of triacylglycerols and HDL-cholesterol. Importantly, linoleic acid (arachidonic acid precursor) changed monocyte/macrophage phagocytosis only to a small extent. Different intensities of ERK1/2 kinase phosphorylation were noted in the environments of arachidonic acid and linoleic acid.

Conclusion: Monocyte/macrophage phagocytosis is a phenomenon dependent on many external factors. The intensity of this process is associated with PPAR-γ activation. Phosphorylation is one of the mechanisms of activation/inactivation of PPAR-γ in macrophages. It seems that arachidonic acid, while inhibiting ERK1/2 phosphorylation, may activate PPAR-γ, causing intensified monocyte/macrophage phagocytosis.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/11207 must be provided.

1. Effect of supplementation with conjugated dienes of linoleic acid (CLA), the main components of the preparations for slimming, on the fluoride, calcium and magnesium content in the hard tissues and serum of mice.
2. Fluoride influence on energy synthesis in skeletal muscle cell line CCL136.
3. Fluoride and lead influence on apoptosis processes in liver cell line HepG2.
4. Influence of conjugated linoleic acids on the synthesis of arachidonic and linoleic acid derivatives in macrophages
5. Effect of heavy metals on hydroxyapatite structures change
6. The sensitivity of immortalized hybrid endothelial-cancer cells EA.hy926 to UVC radiation in comparison with human promyelocytic leukaemia HL60 cells
7. Biosynthesis, transformations and metabolic functions of arachidonic acid derivatives in macrophages. Role in the atherosclerotic process.
8. The identification of embryonic-like SSEA⁺ OCT-4⁺ CXCR4⁺ stem cells in adult bone marrow and cord blood
9. Inhibition of erythrocyte phosphoribosyltransferases (APRT and HGPRT) and decreased energy charge value in rats chronically exposed to lead
10. The influence of glucose in the dialysate on the activity of glutathione peroxidase in hemodialyzed patients.
11. The influence of the soya isoflavones received pre- and postnatally on the mineral metabolism and the function of the male reproductive system in rats
12. Selected interactions between chemical elements and biological active compounds.
13. C677T and A1298C MTHFR polymorphisms affect arechin treatment outcome in rheumatoid arthritis
14. Interactions between aluminofluoride complexes (AlF_x) and proline using the methods of molecular modelling.