Dextran and hyaluronan methacrylate based hydrogels as matrices for soft tissue reconstruction

Stephanie Möller 1Jürgen Weisser 1Sabine Kussmann 2Matthias Schnabelrauch 1

1. INNOVENT Technologieentwicklung, Pruessingstrasse 27B, Jena D-07745, Germany
2. Fzmb, Geranienweg 7, Bad Langensalza D-99947, Germany


Polysaccharide hydrogels have become increasingly studied as matrices in soft tissue engineering. Main advantages of these materials are their known cytocompatibility and the possibility to tailor the material properties chemical modification of the educts. In this work cross-linkable polysaccharide methacrylates based on dextran, amino dextrans, and hyaluronan were synthesized and their transformation into stable hydrogels were studied. In comparison to hyaluronan-based materials, the dextran gels showed a reduced water incorporation, better form stability, mechanical strength, and longer durability. The formed gels had no cytotoxic effects against CHO and 3T3 cells, but cells could only adhere inefficiently in long term experiments. Smooth gel surfaces, fibronectin addition and the use of mixed gels improved the adherence of cells. Different scaffold architectures usable as matrices for soft tissue engineering were studied in vitro including porous and perforated gels and a layer-by-layer assembly in which each layer was overgrown with cells. Embedding of cells between two layers inhibited their aggregation to spheroids and cells kept attached at the support. Selected hydrogels were examined in a rabbit model in vivo to study their biocompatibility, stability, and degradation. No signs of inflammation were seen and with prolonged duration the material was degraded and lacunas were formed obviously by immigrating or ingrowing cells. Additionally, in dextran gels an ingrowth of cells from the surrounding into little channels could be observed similar to the sprouting of small vessels. Optimizing their mechanical properties, the dextran hydrogels represent promising candidates as matrices for soft tissue reconstruction.

Legal notice
  • Legal notice:

    Copyrighted materials, (c) Pielaszek Research, all rights reserved.
    The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced, stored in or introduced into a retrieval or caching system, or transmitted in any form or by any means (electronic, mechanical, photocopying, recording or otherwise), or for any purpose, without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
    In every case, proper references including Author(s) name(s) and URL of this webpage: must be provided.


Related papers
  1. Reagentless biosensors based on mediator-modified electrodeposition hydrogels

Presentation: Keynote lecture at E-MRS Fall Meeting 2006, Symposium J, by Matthias Schnabelrauch
See On-line Journal of E-MRS Fall Meeting 2006

Submitted: 2006-05-12 12:52
Revised:   2009-06-07 00:44
© 1998-2021 pielaszek research, all rights reserved Powered by the Conference Engine