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Pressure and Temperature Induced Peculiarities on Blood Serum Catalase Activity

Lyudmila I. Donchenko 1Natalya V. Shishkova 2Vladimir I. Barbashov 2Anatolii V. Stepura 1

1. Donetsk State Medicine University, Scientific Research Institute for Traumatology & Orthopeadics (DonIT&Or), 106, Artema St, Donetsk 83048, Ukraine
2. National Academy of Sciences of Ukraine, A.Galkin Donetsk Institute for Physics & Technology (DonPTI NASU), Roza Luxemburg 72, Donetsk 83114, Ukraine

Abstract

Catalase (E.C.1.11.1.6) from human erythrocytes is heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen.
In this work the effects of high pressure treatment and temperature on human catalase activity have been studied.
The human blood serum was subjected to high hydrostatic pressure in the range 12.5- 300 MPa at various temperatures up to 500C. The catalase activity was determined using the spectroscopy method based on hydrogen peroxide capability to form with Mo-salts the intensive colored complex.
It was revealed that antioxidant effect of blood serum under pressure has cyclic character.
There are some pressure regions where level of catalase activity was increased and decreased.
It was investigated the temperature effect at constant pressure on catalase activity as well. The obtained dependencies of catalase activity have the complex character.
The revealed results will be discussed in the work.

 

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Related papers

Presentation: poster at E-MRS Fall Meeting 2003, High Pressure School 2003 (5th), by Lyudmila I. Donchenko
See On-line Journal of E-MRS Fall Meeting 2003

Submitted: 2003-05-07 17:52
Revised:   2009-06-08 12:55