The influence of synthesis conditions on properties of porous epoxycontaining hydrogels obtained by radical copolymerization of 2-hydroxyethylamine and epoxycontaining monomers of allylglycidilic ether and glycidilmethacrylate in the presence of cross-linking agent in water frozen systems was studied.
N, N'-methylenebisacrylamide (MBAA) was used as cross-linking agent. By varying of its abundance in the base mixture we can obtain gels with the different ability for swelling and penetrability.
Realization of the gel synthesis in water solution at low temperature (-15C - -20C) allows attempting of carrier high porosity. Gels obtained at lower temperature (-30C) have lower penetrability and better swelling.
Increased concentration of initiator regarding to the monomer mixture leads to the higher product yield.
Due to the porous structure features gels are stable in the solutions with different ionic force. Microscopic study of gels shows that they contain transparent pores with the size of tens and hundreds of microns according to synthesis conditions.
Studying of synthesized polymer systems in water saturated condition by structure-dynamic method shows that with the application of load we can observe two deformation areas, first for extrusion from macropores and second for deformation of cross-linked areas.
Using this data the distances between polymer phase lacing knots were calculated. Their values lay between 20000-60000 D.
Presence of highly reactive epoxy groups in polymers enables the immobilization of different proteins in mild conditions without extra activation of the carrier.
Studying of the immobilization conditions influence on the amount of immobilized albumin allows discovering of optimal process parameters and shows that amount of immobilized protein reaches 150 mg/g of the carrier.

References
Shtilman M.I. Immobilization on polymers.-VSP: Utreht-Tokyo-1994.-479p.

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