Intestinal subepithelial myofibroblasts, located in the lamina propria under the epithelial cell layer, have the specific functions in the gastrointestinal tract. Many of the factors are secreted by the activated myofibroblasts in the intestine in various disease states: experimental colitis, small bowel injury, gastric ulcer models or disease and also in naturally occurring inflammatory bowel disease (IBD). IL-8, one of the factors secreted by the activated myofibroblasts in the intestine, plays sequential role in neutrophil recruitment in the inflamed tissue. In the last few years, the increasing production of electromagnetic (EMF) and static magnetic fields (SMF), due to the expanding use of electronic devices in everyday life, has led to a number of studies on the effects of these fields on living organisms. EMF therapy, because of its anti-inflammatory properties, may be used in medicine in IBD treatment. This mechanism has not been elucidated yet.
In the present work normal human colon myofibroblasts CCD-18Co were exposed to SMF with a flux density of 300 mT. After 24 and 48 hours incubation TNF-α-dependent IL-8 secretion was determined with ELISA kit (R&D Systems) and the cell viability was determined with TOX-2 (In Vitro Toxicology Assay Kit XTT Based, TOX-2, Sigma).
It was shown that SMF has no effect on TNF-α-dependent IL-8 secretion and cell viability after 24 and 48 hours. This work was supported by the Medical University of Silesia (KNW-2-017/N/3/N).

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