Glycosyltransferases are very important proteins. They play a crucial role by participating in biosynthesis of glycolipids, glycoproteins, oligosaccharides and other glycoconjugates.  They are responsible for synthesis of cell surface glycoconjugates. Galactosyltransferases assist in biosynthesis such important glycoconjugates as blood group antigens, Lewis antigens and lipopolysaccharides, therefore they are promising target for antiviral and anticancer drugs  Study of enzymatic activities and inhibition of GTs are of considerable interest of many works in this field of science. Such study indicate possible way towards a suitable tool to measure the specific activity of potent antiviral or antitumour agents towards the mammalian source of GTs.

This communication describes the isolation of soluble glycosyltransferases from swine tissues. In our experiments, various  tissues were tested – lymph nodes, thymus and liver. Performed study allow to reduce the isolation procedure time, offering promising prospects for preparation of this kind of enzyme from mammalian source – swine tissue.   

Acknowledgement

Research studies part-financed by the European Union within the European Regional Development Fund (POIG.01.01.02-14-102/09).

References:

[1] Wandzik I., Bieg T., Czaplicka M., „Synthesis of 2-deoxy-hexopyranocyl derivatives of uridine as donor substrate analogues for galactosyltransferases”, Bioorg. Chem., 2009, 37, 211-216.

[2]  Berg E. G., Rohrer J., „Galactosyltransferase—still up and running”, Biochimie, 2003, 85, 261–274.

[3] Breton C., Mucha J., Jeanneau C., „Structural and functional features of glycosyltrasferases”, Biochimie, 2001, 83, 713-718.

[4]  Breton C., Imberty A., „Structure/function studies of glycosyltransferases”, Cur. Opin. Struct. Biol., 1999, 9, 563–571.

[5]  Descroix K., Pesnot T., Yoshimura Y., Gehrke S., Wakarchuk W., Palcic M. M., Wagner G. K., „Inhibition of Galactosyltransferases by a Novel Class of Donor Analogues”, J. Med. Chem., 2012, 55, 2015−2024

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