The antiproliferative effect of pterostilbene on colon cancer cells in vitro

Joanna M. Wawszczyk ,  Małgorzata Kapral ,  Andrzej Hollek ,  Ludmiła Węglarz 

Department of Biochemistry, Medical University of Silesia, Jedności 8, Sosnowiec 41-200, Poland

Abstract

Colon cancer remains the second leading cause of cancer mortality in Poland in the last years. Epidemiological studies showed that dietary phytochemicals may exert chemopreventive and therapeutic effect against colorectal cancer. There is a growing interest in identifying new biologically active agents from dietary sources in this respect. Pterostilbene (trans-3,5-dimethoxy-4-hydroxystilbene) is a naturally occurring stilbene, that has been found to have antioxidant, anti-inflammatory and antiproliferative properties. The greater bioavailability of pterostilbene than other stilbenes indicated that it could be potentially developed for clinical applications. Recent studies showed that pterostilbene exhibits the hallmark characteristics of an effective anticancer agent based on its antineoplastic properties in several common cancers.

The aim of this study was to analyze antiproliferative and cytotoxic effects of pterostilbene on human colon cancer cells Caco-2. They were cultured using standard techniques and exposed to increasing doses of pterostilbene (5-100 mM) for 48 and 72 h. Cell proliferation was determined by sulforhodamine B assay. The growth of treated cells was expressed as a percentage of untreated control cells. Pterostilbene decreased proliferation rate of Caco-2 cells in a dose- and time-dependent manner. Its concentrations ≤ 25 mM did not affect cell growth for 48 hours treatment. Significant growth inhibition was observed in cell cultures incubated with higher concentrations of pterostilbene (40-100 mM). The prolongation of cells exposure to stilbenoid at all concentrations used (5-100 mM) up to 72 h resulted in their considerable growth inhibition. The maximum growth reduction was observed at 100 mM pterostilbene (73-78% of control).

The findings of this study showed significant antiproliferative effects of pterostilbene against human colon cancer cells in vitro.

 

Legal notice
  • Legal notice:

    Copyright (c) Pielaszek Research, all rights reserved.
    The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
    In every case, proper references including Author(s) name(s) and URL of this webpage: http://science24.com/paper/31047 must be provided.

 

Related papers
  1. Detection of the poor metabolizer-associated CYP2D6 gene by long- and tetra-primer PCR technology
  2. Role of gene polymorphism in the optimalisation of the treatment with vitamin K antagonist. 
  3. The effect of sulphasalazine and 5-aminosalicylic acid on the human colon myofibroblasts
  4. Antiproliferative activity of inositol hexaphosphate against human skin melanoma cells
  5. The effect of inositol hexaphosphate on the transcriptional activity of genes encoding cyclin D1 and histone H3 in human intestinal cells stimulated with interleukin 1β
  6. Inhibition of inducible nitric oxide synthase expression by inositol hexaphosphate in cultured human colon cancer cells exposed to proinflammatory agents
  7. Effect of phytic acid on the expression of IL-6, IL-8 and NFkB in human colonic epithelial cells treated with IL-1β  
  8. The influence of TNF-α (tumor necrosis factor - alpha) on concentration of soluble adhesion molecules in cultures of HT-29 cells exposed to inositol hexaphosphate (IP6)
  9. Phytic acid down-regulates IL-8 secretion from IL-1β stimulated colonic epithelial cells by influencing mitogen-activated protein kinase signaling pathway.
  10. Impact of celecoxib on soluble intercellular adhesion molecule-1 (sICAM-1) and soluble E-cadherin (sE-cadherin) concentrations in human colon cancer cell line cultures exposed to phytic acid (IP6) and TNF-α (tumor necrosis factor-alpha)
  11. The effect of inositol hexaphosphate on the transcriptional activity of genes encoding metalloproteinases and their tissue inhibitors in colon cancer cellsstimulated with phorbol-12-myristate 13-acetate
  12. Modulation of the expression of genes encoding TGF-β isoforms and their receptors by inositol hexaphosphate in human colon cancer cells
  13. Influence of troglitazone, sodium butyrate and 5-aminosalicylic acid on the chemokine ENA-78/CXCL5 secretion in the intestinal subepithelial myofibroblasts
  14. The chelation ability of chosen metal ions by protective ointments containing Na2H2EDTA  and aminoacid
  15. Microsatellite markers analysis in the treatment and diagnosis of familial hypertrophic cardiomyopathy
  16. The influence of inositol hexaphosphate on the expression of genes encoding matrix metalloproteinases 2 and 9 and their tissue inhibitors in human colon cancer cells
  17. Evaluation of transcriptional activity of genes encoding IL-6 and its receptor in colon cancer cells treated with phytic acid
  18. The effect of inositol hexaphosphate on NFkB expression in human colon cancer cells
  19. Transcriptional activity of interferon γ and two subunits of its receptor as molecular markers of myocarditis
  20. Expression of matrix metalloproteinase 2 and matrix metalloproteinase 9 in laryngeal squamous cell carcinoma
  21. Expression of genes of tumor necrosis factor -α and its receptors in colon cancer Caco-2 cells treated with inositol hexaphosphate
  22. The influence of phytic acid on NFκB expression in human colon cancer cells
  23. The degree of substitution of Desulfovibrio desulfuricans lipopolysaccharides and their lipid A component by phosphates
  24. The comparison of free and melanin-bound lipofuscin isolated from pig iris
  25. GROWTH OF HUMAN CHONDROCYTES ON BIODEGRADABLE SYNTHETIC POLYMERS
  26. INFLUENCE OF SODIUM BUTYRATE ON ANTIOXIDATIVE ENZYMES ACTIVITY IN CACO-2 CELL LINES.
  27. ANTI-PROLIFERATIVE EFFECTS OF INOSITOL HEXAPHOSPHATE AND VERAPAMIL ON HUMAN COLON CANCER CACO-2 AND HT-29 CELLS

Presentation: Poster at IX Multidyscyplinarna Konferencja Nauki o Leku, by Joanna M. Wawszczyk
See On-line Journal of IX Multidyscyplinarna Konferencja Nauki o Leku

Submitted: 2014-03-12 10:31
Revised:   2014-05-02 19:32