Bioactive glass-modified composites for bone tissue engineering
|Joanna K. Filipowska 1, Magdalena K. Widziołek 2, Justyna Kokoszka 3, Katarzyna Cholewa-Kowalska 4, Grzegorz J. Tylko 2, Elzbieta Pamula 4, Maria Łączka 4, Anna M. Osyczka 2|
1. Klinika Ortopedii i Traumatologii Narządu Ruchu, Instytut Fizjoterapii, UJ CM, os. Złotej jesieni 1, Kraków 31-826, Poland
Artificial bone substitutes include metals, polymers and ceramics that are selected for clinics by their ability to support bone cell growth and osteogenic differentiation of progenitor cells, i.e. they display osteoconductive properties. Yet, one-phase materials are not able to fulfil the requirements for the ideal bone substitute and very few biomaterials are osteoinductive, i.e. they induce osteogenesis on their own. We have tested the hypothesis the incorporation of gel-derived bioactive glasses (SBG) into polymer (i.e. poly(L-lactide-co-glycolide; PLGA) or titanium dioxide (TiO2) will modify the nanostructure of resulting composite materials, improve their mechanical properties, surface activity (i.e. bioactivity) and result in composite osteoinductivity in human mesenchymal stem cell (hMSC) cultures. Composites made of either TiO2 or PLGA were combined with either A2 or S2 SBG of respective CaO/SiO2 ratios: 54/40mol% (A2) or 16/80mol% (S2). PLGA was combined with 21%vol of A2 or S2 S-BG, whereas TiO2 with 25, 50 or 75 wt% of A2 or S2 SBG. PLGA-SBG composites were next fabricated into 3D scaffolds, whereas TiO2-SBG composites into cell growth surfaces. Human MSC were harvested from bone marrow of adult patients, seeded onto scaffolds and surfaces and stimulated in culture with either dexamethasone (Dex) or recombinant human BMP-2 up to 20 days. Bone-like hydroxyapatite structures formed mainly inside the PLGA-SBG scaffolds. Human MSC cultures on bioactive PLGA-A2 scaffolds increased ALP activity, osteopontin and BMP-2 mRNAs without Dex or BMP-2 treatments. When hMSC on PLGA-A2 scaffolds were treated with BMP-2, cells elevated ALP activity, RANK-L, osteoprotegrin and osteocalcin mRNAs compared to plain PLGA.
Presentation: Poster at Nano-Biotechnologia PL, by Joanna K. Filipowska
See On-line Journal of Nano-Biotechnologia PL
Submitted: 2012-06-30 21:11 Revised: 2012-06-30 21:48
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