The influence of platinum nanoparticles on human epidermal keratinocytes. 

Anna Goralczyk 1Radoslaw Szmyd 1Lukasz Skalniak 1Agnieszka Cierniak 1Barbara Lipert 1Francesca L. Filon 2Matteo Crosera 2Julia Borowczyk 1Eliza Laczna 1Justyna Drukala 1Andrzej Klein 1Jolanta Jura 1

1. Jagiellonian University, Faculty of Biochemistry, Biophysics and Biotechnology, Kraków 30-387, Poland
2. Università di Trieste, Trieste, Italy

Abstract

Nanoparticles (NPs) have diverse applications in industry, medical devices, therapeutic agents and cosmetics. Nanoparticles are defined as a structures that have at least one dimension of nanometer scale. The unique physicochemical properties of engineered nanoparticles are attributable to their small size, large surface area, chemical composition, surface reactivity, charge, shape and media interactions. Although impressive from the perspective of material science, the novel properties of NPs could lead to adverse biological effects with the potential to create toxicity. Because of their strong catalytic activity, platinum nanoparticles are commonly used in converters of automobiles causing emission of PtNPs to the environment. In recent years PtNPs have attracted even more attention due to the fact that they have been shown to quench reactive oxygen species. They are added to supplements or cosmetics due to their anti-oxidative and anti-aging properties. While the commercialization of platinum nanoparticles expands rapidly, their impact on health and environment is still not well understood. In this study, the cellular influences induced by 4-5 nm polyvinylpyrrolidone-coated PtNPs on human primary keratinocytes was investigated. Cytotoxicity, genotoxicity, morphology, metabolic activity and changes in the expression of proteins were studied in PtNPs- treated cells. Also the antimicrobial properties of PtNPs were verified. My studies show, that despite the positive effects, nanoparticles may present possible danger, concerning activation of genotoxic processes, although no cytotoxic effects were observed. It was also found out that PtNPs are effective against Gram- negative bacteria. This data might contribute to better understanding of effects triggered by PtNPs on cells and may provide valuable information to develop safety regulations for the use of nanoparticles in various applications.

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Presentation: Poster at Nano-Biotechnologia PL, by Anna Goralczyk
See On-line Journal of Nano-Biotechnologia PL

Submitted: 2012-06-30 19:36
Revised:   2012-08-14 09:41
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