This paper is a presentation of our results from studies based on a development of novel liposomal nanocarriers which can be applied in delivery of many biologically substances such as proteins or antiarrhythmic drugs like phenytoin. There is a appreciable interest in liposomal carriers which could be used for medical and pharmacological applications.  Because of low stability of liposomes these applications are challenging. Some external factors such as pH, temperature or other substances present in an environment  may have damaging effect on the liposomes.

Proteins are known to play very important role  in functioning of  living organisms. They have  mechanical-structural functions but also they can act as biocatalysts (enzymes), neurotransmitters or can be involved in enabling  transport of  small molecules and ions in tissue.  Alkaline phosphatase [ALP], belonging to the class of  hydrolases, was choosen as model protein. ALP is responsible for the dephosphorylation processes of various  compounds (including  proteins).  The highest activity of ALP is strictly related to high pH of solutions. The main sources of alkaline phosphatase are: liver, bone tissues, kidneys or placenta. Due to this fact, ALP can be used as a biomarker. That’s why it can be used in diagnostic for some diseases. Moreover, alkaline phosphatase is related to biomineralization process.

Other kind of encapsulated drug was phenytoin. It was used as a model drug which can be used for reducting the electrical conductivity of the brain cells. But one of the most important application is being an antiarrhythmic agent. Due to low solubility in agueous media and high toxicity, phenytoin is delivered into liposomal bilayer.

Because of low stability of liposomes, they were coated by derivatives of poli(vinyl alcohol) [PVA]. It was choosen due to good solubility in water, high resistance to organic solvents and wide use in cosmetic or pharmaceutical applications. We obtained the hydrophobic and ionic derivatives of PVA to use them later to coat the outer surface of vesicles.

Acknowledges:

The study was conducted according to the TEAM Foundation for Polish Science, co-funded by European Regional Development Fund: PolyMed, TEAM/2008-2/6.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/28299 must be provided.