Advanced microtechnologies for cytogenetic analysis

Dorota Kwasny 1Indumathi Vedarethinam Pranjul Shah Maria Dimaki Asli Silahtaroglu Zeynep Tumer Winnie E. Svendsen 

1. Technical University of Denmark (DTU), Oersteds Plads, Copenhagen 2800, Denmark

Abstract

Cytogenetic and molecular cytogenetic analyses, which aim to detect chromosome abnormalities, are routinely performed in cytogenetic laboratories all over the world. Traditional cytogenetic studies are performed by analyzing the banding pattern of chromosomes, and are complemented by molecular cytogenetic techniques such as fluorescent in situ hybridization (FISH). To improve FISH application in cytogenetic analysis the issues with long experimental time, high volumes of expensive reagents and requirement for trained technicians need to be addressed. The protocol has recently evolved towards on chip detection of chromosome abnormalities with the development of microsystems for FISH analysis. The challenges addressed by the developed microsystems are mainly the automation of the assay performance, reduction in probe volume, as well as reduction of assay time.

We present here our efforts to introduce automation in the cytogenetic laboratories at a microscale. We have developed membrane based micro perfusion systems capable of expansion of lymphocytes in a shorter time and at a smaller scale. The simulated and experimental results show very efficient exchange of the growth medium to the hypotonic solution and fixative. These are commonly used solutions required for proper preparation of a metaphase chromosomes analysis. Further we developed a microfluidic chip for preparation of metaphase chromosome spreads and their analysis by metaphase FISH on chip. All developed devices are capable of performing the entire metaphase FISH protocol in a shorter time and at the same quality as standard methods.

Auxiliary resources (full texts, presentations, posters, etc.)
  1. PRESENTATION: Advanced microtechnologies for cytogenetic analysis, Microsoft Office Document, 10MB
 

Legal notice
  • Legal notice:

    Copyright (c) Pielaszek Research, all rights reserved.
    The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
    In every case, proper references including Author(s) name(s) and URL of this webpage: http://science24.com/paper/28240 must be provided.

 

Presentation: Oral at Nano-Biotechnologia PL, by Dorota Kwasny
See On-line Journal of Nano-Biotechnologia PL

Submitted: 2012-06-23 19:54
Revised:   2012-06-25 15:09
Google
 
Web science24.com
© 1998-2022 pielaszek research, all rights reserved Powered by the Conference Engine