Present-day antitumor drugs act by killing cells that divide rapidly, one of the main properties of most cancer cells. This means that they are also toxic to cells that divide rapidly under normal circumstances and cause severe side effects. The aim of researches on new substances is to find a derivative with similar antitumor activity to applied drugs but with better selectivity.

A novel derivative was obtained in reaction of N-(pyridin-2-yl)picolinoamidrazone with cis-1,2,3,6-tetrahydrophthalic anhydride and its Cu(II) complex which was created in reaction with copper (II) acetate. New structures were confirmed with IR and NMR spectroscopy, elemental and  X-ray diffraction  analyses. 

The compounds were evaluated in vitro against Gram-negative and Gram-positive bacterial strains to assess their antimicrobial activity. The MIC­­₅₀ values (the minimal inhibitory concentration required to inhibit the growth of 50% of organisms) ranged from 90 to 400µg/mL for the free ligand and from 50 to 250µg//mL for copper complex. Both tested substances had a relatively low antibacterial activity, although the potency of the complex with Cu was better than the activity of the free ligand.

In cytotoxicity research the ligand shown anti-inflammatory effect by inhibition of IL-6 synthesis. Cu (II) complex in concentration 100m/ml displayed strong antiproliferative activity against tumor lines SW 948, CX-1 and A-431 comparable to cisplatin used as a reference drug. Morover copper complex posses low cell toxicity and shown no TNF-α inhibition which makes it interesting substance for potential antitumor drug.     

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