Cefuroxime, (6R,7R)-3-{[(aminocarbonyl)oxy]methyl}-7-{[(2E)-2- (2-furyl)- 2(methoxy- imino)acetyl]amino}-8-oxo-5-thia- 1-aza- bicyclo[4.2.0]oct-2-ene-2-carboxylic acid is a second-generation cephalosporin antibiotic. Although as a second-generation it is less susceptible to ß-lactamase and and so may have greater activity against Haemophilus influenzae, Neisseria gonorrhoeae and Lyme disease. Unlike other second generation cephalosporins cefuroxime can cross the blood-brain-barrier.

A HPLC method with UV detection for the determination of cefuroxime in human plasma has been developed. The method used cefalexin as the internal standard. The drug and the internal standard were isolated from plasma using a protein precipitation. The analysis was carried out on Supelcosil LC-18-DB 250 x 4.6 mm, 5 µm (Supelco) column using a 0.07 M sodium dihydrophosphate solution containing 10 % acetonitrile as the mobile phase. The flow rate was 1.7 mL/min. The linearity was established over the concentration range of 0.2 – 12.0 µg/mL. The method was validated according to FDA and EMEA requirements and all of the investigated parameters met the acceptance criteria.

The developed method can be applied to the pharmacokinetic studies in humans, e.g. bioequivalence studies.

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