Oxidation of glucose at nanostructured composite bioelectrocatalytic systems

Marta Gierwatowska ,  Barbara Kowalewska ,  Paweł J. Kulesza 

University of Warsaw, Faculty of Chemistry, Pasteura1, Warsaw 02-093, Poland

Abstract

We propose new bioelectrocatalytic system containing carbon nanotubes stabilized with 4-(pyrrole-1-yl) benzoic acid (PyBA) active towards glucose oxidation.

The presence of 4-(pyrrole-1-yl) benzoic acid in such integrated systems improves their stability and introduces new functional (carboxyl) groups which play an important role in the enzyme immobilization on surfaces of such layers. Systems with controlled composition and physicochemical properties act as good carriers and mediators for enzymes and other catalytic redox centres.

As a redox mediator, tetrathiafulvalene (TTF) has been used. The presence of TTF facilitates the effective flow of electrons from redox centres of the enzyme (glucose oxidase) to the glassy carbon electrode. Carbon nanotubes in such systems plays an important role, forming a three-dimensional "nanowire” network around the enzyme molecule, as well as they improve electronic conductivity of the bioelectrocatalytic layer. Combination of glucose oxidase, carbon nanotubes and the appropriate redox mediator has produced a system capable of effective oxidation of glucose in 0.1M phosphate buffer (pH = 7.0).

The problem of hydrogen peroxide (which is a product of glucose oxidation) has also been addressed. The use of horseradish peroxidase (HRP) and catalase (CAT), i.e. enzymes capable of H2O2 decomposition has led to increase of the overall catalytic activity during electrooxidation of glucose and shifted the oxidation potentials towards more negative values.

The proposed integrated electro-active systems towards glucose oxidation in neutral environment seem to be of great importance to the development of biofuel cells.

 

Related papers
  1. Development of multifunctional bioelectrocatalytic films for oxidation of ethanol   
  2. Integrated carbon nanotube based mediating systems for bioelectrocatalysis: application to oxygen reduction and glucose oxidation
  3. Preparation of copper hexacyanoferrate multilayer films modified with 4-(Pyrrole-1-yl) benzoic acid on glassy carbon electrode.
  4. Development of electrocatalytic materials based on the mixed addenda Dawson heteropolyanion and conducting polymers
  5. Development of novel bioelectrocatalytic systems through controlled combination of multiwalled carbon nanotubes, redox mediators and enzymes
  6. Application of Inorganic Redox - Conducting Solids As Charge Relays in Dye-Sensitized Solar Cell
  7. Bi-functional electrocatalytic systems for oxygen reduction in acid medium
  8. Incorporation of RuSex/C within nanostructural Ir matrices to enhance oxygen reduction
  9. Electrochemical charging of carbon nanotubes modified with polyoxometallates monolayers
  10. Enzymatic carbon nanotube based composite electrodes for dioxygen reduction
  11. Multifunctional bio-electrocatalytic systems for reduction of oxygen and hydrogen peroxide
  12. Electrocatalysis and Bioelectrocatalysis at Network Films of Metal Nanoparticles and Carbon Nanostructures
  13. Bioelectrocatalytic dioxygen reduction at carbon nanotubes – silicate composite film modified electrode
  14. Development and characterization of bioelectrocatalytic systems for oxygen reduction
  15. Activation of Methanol-Tolerant Carbon-Supported RuSex Electrocatalytic Nanoparticles Towards More Efficient Oxygen Reduction
  16. Electrocatalysis and bioelectrocatalysis and nanostructured composite films
  17. New strategies in the electroctalytic reduction of oxygen for fuel and biofuel cells
  18. Polyoxometallate-modified conducting polymer linked Pt nanoparticles as bifunctional electrocatalysts for bromate reduction
  19. Network Films of Conducting Polymer Linked and Polymeatallate Stabilized Platinum Nanoparticles

Presentation: Poster at SMCBS'2009 International Workshop, by Marta Gierwatowska
See On-line Journal of SMCBS'2009 International Workshop

Submitted: 2009-09-07 19:53
Revised:   2009-09-07 19:59