Electrochemistry of redox-labelled DNAs immobilized onto electrodes through the de-novo designed triazene linker 

Elahe Farjami ,  Majken Hansen ,  Elena E. Ferapontova ,  Kurt Gothelf 

University of Aarhus, Chemistry Department, Center for DNA Nanotechnology (CDNA), Langelandsgade 140, Aarhus 8000, Denmark


Redox-labeled DNA was conjugated to and grafted to carbon and gold electrodes using the newly synthesized triazene linker. The chemical and electrochemical activation and deposition steps included using Me2SO4 reducing agent and further potentiostatically controlled deposition procedure. The produced DNA-modified electrodes exhibit remarkable DNA-hybridization properties which can be detected by a shift in the redox potential and they can easily be renewed by repeated hybridization/de-hybridization procedures. Electrochemical studies of surface coverage and DNA hybridization efficiency demonstrated that grafting through the triazene linker can successfully compete with existing protocols for DNA immobilization through the commonly used alkanthiol linkers and diazonium salts, while giving the practical advantage of one-step immobilization procedure. 


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Presentation: Short communication at SMCBS'2009 International Workshop, by Elahe Farjami
See On-line Journal of SMCBS'2009 International Workshop

Submitted: 2009-08-30 14:13
Revised:   2009-09-03 09:35