Non-cytotoxic small Carbon Nano-Onions - the first covalent functionalization with biomolecules

Marta E. Płońska-Brzezińska 1Joanna Łuszczyn 1Amit Palkar 2Alina Dubis 1Agneta Simionescu 2Dan T. Siomionescu 2Beata Kalska-Szostko 1Luis Echegoyen 2

1. University of Białystok, Institute of Chemistry, Hurtowa 1, Białystok 15-399, Poland
2. Clemson University (CLEMSON), Clemson, SC, Clemson 29634, United States


Application of small (6 to 8 shells) carbon nano-onions modified with the carboxylate groups (ox-CNOs) in biosensor construction were performed. Main attention is paid to study the ability of modified CNOs to interact with other functionalized biologically active compounds.

Carbon nano-onions were prepared and functionalized with carboxylic group by chemical oxidation and by pegylation using method applied by Lieber et al. for surface modification of SWNTs.1 These nanostructures after functionalization become soluble in aqueous solutions. MTS test showed excellent cytocompatibility of all CNOs tested at 30 and 3000 μg/ml. Therefore these carbon nanostructures can be safely used for intended biological applications. For the first time covalent functionalization of ox-CNOs with biomolecules has been reported. It was used biotin-avidin interaction. The oxidized CNOs particles were immobilized by amidation reaction on Au surface.

Multilayers were prepared on gold surface by layer-by-layer assembly method and the process was monitored by surface plasmon resonance (SPR) and atomic force microscopy (AFM). The Fourier transform infrared spectroscopy with total attenuated reflactance mode (FT-IR/HATR) were also used to characterize the multilayer formation. The FT-IR spectroscopy confirmed covalent linkage between interacting molecules.

In conclusion, functionalized small CNOs (5-6nm) can be potentially used for developing chemical sensors and biosensors.

[1]  Wong, S.; Woolley, A.; Joselevich, E.; Cheung, C.; Lieber, C. J. Am. Chem. Soc. 1998, 120, 8557

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Presentation: Poster at E-MRS Fall Meeting 2009, Symposium F, by Marta E. Płońska-Brzezińska
See On-line Journal of E-MRS Fall Meeting 2009

Submitted: 2009-05-22 09:31
Revised:   2009-06-07 00:48
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