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Response of Cell Types to Inkjet Printing

Brian Derby 

University of Manchester - School of Materials Science - Materials Science Centre, grosvenor street, Manchester M1 7HS, United Kingdom

Abstract

Inkjet printing provides a versatile method for the simultaneous fabrication of tissue scaffold structures and parallel seeding. One of the potential benefits of this technology is the ability to fabricate structures with heterogeneous cell populations, with distinct cell types at specific locations within a printed structure. Our previous work has already demonstrated that human fibroblast cells can be successfully dispensed using commercial inkjet printing systems with cell death rates indistinguishable from that of control cultures [1]. However, in order for inkjet printing to be a viable technique for cell dispensing, we must be sure that it can be used with a range of cell types without damage. Here we present a survey of cell behaviour after inkjet printing of primary human fibroblasts and osteoblasts, fibroblast and oral keratinocyte cell lines and primary bovine chondrocytes. All cell types are shown to be printable and that conditions can be identified to minimise cell death after printing to levels that are indistinguishable from unprinted controls. Osteoblast and chondrocyte cells appear to show a response to printing that affects either their proliferation or metabolic rate after printing that is not seen with fibroblasts or keratinocytes. It is hypothesised that this may reflect the stress sensitivity of these cell types.

 

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Related papers

Presentation: Invited oral at E-MRS Fall Meeting 2008, Symposium L, by Brian Derby
See On-line Journal of E-MRS Fall Meeting 2008

Submitted: 2008-07-28 10:08
Revised:   2009-06-07 00:48