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OWLS See in the Darkness: a Label Free Microbiological Detection Method with SiTiO and InSnO Coated Planar Waveguide Sensor

Károly Somogyi 1Istvan Szendro 1Katalin Erdélyi Zsolt Puskás Márk Fábián 

1. MicroVacuum, Kerékgyarto u. 10, Budapest H-1147, Hungary

Abstract

The surface sensitivity of an optical waveguide is higher in the case of thin planar waveguides with a greater specific surface. The evanescent field of the light will detect the surface coating. Such optical waveguide lightmode sensors were developed on the basis of transparent SixTi1-xO2 layers with the necessary grating and can be successfully operated both in inorganic chemistry and in life sciences as label free biosensors. On the other hand, voltammetric and/or potentiometric electrochemical measurements also play paramount importance in the same and similar investigations. Since the optical waveguide lightmode detection method allows a label free approach in microbiological investigations with high sensitivity, presently the need of the combination of these mentioned two methods is of vital importance.

SixTi1-xO2 is a dielectric and the electrochemical measurements require a conducting electrode, thus, optically transparent, but conductive thin layer must be deposited on the grated optical sensor. Basically indium-tin-oxide (ITO) nanolayer covered chips were developed. At about 10 nm thick ITO layers show sufficiently good electrical conductivity and their good optical transparency corresponds to the requirements of the optical sensing. Outstanding sensibility of the ITO coated sensor chips on any surface modifications allows a highly attracting application of these layers.

It was demonstrated that the presence of the electrical field in an electrochemical cuvette incorporated into an integrated measuring instrument affects both the transport and the adsorption/desorption processes of the investigated species at the surface of the sensor. Lactic acid bacteria were adsorbed in native form on the surface of the sensor chips by ensuring polarizing potential (1 V) and were exposed to various chemical stressors and the behaviour of bacteria was monitored.

 

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Related papers

Presentation: Oral at E-MRS Fall Meeting 2008, Symposium B, by Károly Somogyi
See On-line Journal of E-MRS Fall Meeting 2008

Submitted: 2008-05-18 23:47
Revised:   2009-06-07 00:48