Hydroxyapatite and Bioglasses are important bioactive materials as they exhibit direct bonding with human bone. Therefore they are used as a coatings for metallic implants.  The aim of the study was to compare two types of layers: hydroxyapatite (HA) and hydroxyapatite mixed with bioglass (BG) (HA+BG, 50% of BG) during initial stage of their interaction with cell medium. The layers were deposited by pulsed laser deposition technique on 316L stainless steel with nanocrystalline diamond as a buffer film. The in vitro study was performed in order to determine the effect of the two layers on the cells response. Human osteoblast-like cells (MG-63) were cultured on examined surfaces and on polystyrene commercial 48-well cell culture plates (TCPS) used as a control. After 3 and 7 days the behaviour of the cells grown on different surfaces was compared through determination of cell adhesion (CV colorimetric assay). Cell morphology and properties of biomaterials surfaces were analysed by atomic force microscopy (AFM).

The results of CV tests performed after 3 days of culture proved the much better ability of HA/BG layers to stimulate cell adhesion in its initial stage compared to HA and TCPS. After 7 days’ culture the results of CV tests didn’t differ significantly for HA and HA+BG but were still much better than in the case of TCPS.

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