The important problem of scientific and applied character is a study
of serum reactions, allowing to understand a new aspects of
pathogenesis of diseases connected with autoimmune processes in an
organism. In this respect the demonstrative model is a process of
circulating immune complex (the CIC) formation. Interaction of
antigen- antibody (AG-AB) develops in vivo and in vitro depending on
physico-chemical properties of antigens and antibodies and
experimental conditions. For the last years the significant effect of
high pressure on AG-AB interaction has been revealed, as the forces
included in the AG-AB binding have shown a sensibility to the pressure
[1,2].
In this report we discuss the high hydrostatic pressure (HHP) effect
on peculiarities of the AG-AB binding process in a blood serum
obtained from patients with a mechanical trauma.
Pressure-induced changes in the biological assays were studied both
clinical test - detection of the CIC (by precipitate reaction) and
physico-chemical methods: 1H NMR spectroscopy and pH-solution
determination for initial state and after pressure treatment .
The obtained experimental results indicate that the HHP has rendered
significant effects on the properties of biological fluids under
investigation. The increase of precipitate density values
(activization of serum reaction) is observed for all pressurized
assays in comparison with initial state. However the level of such an
increase was depended on the applied high pressure level.
The values of 1H NMR-spectrum line width (D H ) for the studied assays
are shown that the HHP application to blood sera is accompanied by the
D H increasing for all investigated assays. The wider line corresponds
to lower mobility of hydrogen atoms, and, consequently, the bounds of
hydrogen in system are stronger. The appearance of a new narrower line
(see the point marked by *) indicates that the hydrogen occupies the
two nonequivalent positions in studied system, i.e. the new bounds are
formed. And all these processes can attest to formation of
macromolecular complexes of antigen-antibody.
Thus, at all the investigated pressures we have observed the
increasing of the binding activity of AG-AB in blood serum. Dynamics
of these processes has been determined by HHP level. The higher
activity of CIC process formation in our experiments has observed at
the pressure about 150 MPa.
References
[1] Mozhaev, V.V., Heremans, K., Frank, J, Masson P. and Balny, C.
Trends Biotechnol. 12, 493 (1994)
[2] Green, D.J., Litt ,G.J., Laugharn, J. Clin.Chem.(1998);
44
; 341 (1998)
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