Prussian Blue based planar biosensor for lactate

Eugene Yashina 

Chemistry Department, Moscow State University, Leninskie Gory, 1-3, Moscow 119992, Russian Federation


The construction of sensors is based on the approach for operation of oxidase based biosensors by low potential selective detection of H2O2 on electrodes modified by electrochemically deposited Prussian Blue (ferric hexacyanoferrate). The approach was first demonstrated by our team in 1994 [1,2] and had been developed in order to achieve the most advantageous H2O2 transducer. Electrochemical biosensor for lactate has been made by immobilization of the enzyme lactate oxidase on the top of H2O2 transducer (planar sensor for hydrogen peroxide). Immobilization protocol included a formation of the enzyme containing Nafion polyelectrolyte membrane according to procedure developed earlier [2]. Exposure of lactate oxidase to water-organic mixtures with a high (85%) content of the organic sol­vent, resulted in stabilization of the enzyme by membrane-forming polyelectrolyte. The different approaches affecting the immobilization procedure have been studied. Combining the attractive performance of a Prussian-Blue based hydrogen peroxide transducer and optimal immobilization protocol, we have been developing low cost and mass-productive planar sensors for H2O2 and biosensors for glucose and lactate based on screen-printed electrodes modified with chemically deposited nano-layers of Prussian Blue. The best results were obtained for lactate oxidase immobilized in siloxans membranes. The corresponding biosensor has following analytical characteristics: improved sensitivity (about 70 mA/M cm2) and signal-to-noise ratio, low detection limit to compare with known analogs. Lactate may be analyzed in range of 1.10-6-1.10-3 M in FIA regime. The biosensor retained the sensitivity within 3 days (for 500 measurements) and could be used for multiuse measurements.

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Presentation: Poster at SMCBS'2007 International Workshop, by Eugene Yashina
See On-line Journal of SMCBS'2007 International Workshop

Submitted: 2007-10-07 22:48
Revised:   2009-06-07 00:44
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